Detection of Anti-Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis
نویسندگان
چکیده
منابع مشابه
Detection and analysis of resistance mutations of hepatitis B virus.
OBJECTIVE To investigate the relationships of resistance mutations of hepatitis B virus (HBV) with replication and genotypes of HBV and to understand the common resistance mutations and mutation pattern. METHODS The mutation patterns related to resistance to nucleoside drugs were analyzed, and the relationships of resistance mutations with HBV genotypes, Ct value, HBeAg, alanine aminotransfer...
متن کاملRapid and Reliable Detection of Nonsyndromic Hearing Loss Mutations by Multicolor Melting Curve Analysis
Hearing loss is a common birth defect worldwide. The GJB2, SLC26A4, MT-RNR1 and MT-TS1 genes have been reported as major pathogenic genes in nonsyndromic hearing loss. Early genetic screening is recommended to minimize the incidence of hearing loss. We hereby described a multicolor melting curve analysis (MMCA)-based assay for simultaneous detection of 12 prevalent nonsyndromic hearing loss-rel...
متن کاملCOLD-PCR for early detection of hepatitis B virus antiviral drug resistance mutations.
Nucleos(t)ide analogues (NAs) are effective therapeutic agents for the treatment of chronic hepatitis B virus (HBV) infection. However, longterm use of NAs is often hampered by the emergence of drug resistance mutations, causing potentially serious consequences such as liver decompensation and mortality. A more sensitive method for early detection of drug resistance mutations is needed. Convent...
متن کاملAnti-Hepatitis B Virus Activity of Flavonoids from Marchantia convoluta
Flavonoids from Marchantia convoluta (MCF) were studied for their ability to inhibit the proliferation of 2.2.15 cells. All concentration of MCF inhibited the secretion of HBsAg and HBeAg in the cultured medium by 2.2.15 cells. Analysis of morphological changes of MCF-treated cells by phase-contrast microscopy revealed a possible mode of action for MCF: inhibition of proliferation of 2.2.15 cel...
متن کاملCompensatory variances of drug-induced hepatitis B virus YMDD mutations
Although the drug-induced mutations of HBV have been ever documented, the evolutionary mechanism is still obscure. To deeply reveal molecular characters of HBV evolution under the special condition, here we made a comprehensive investigation of the molecular variation of the 3432 wild-type sequences and 439 YMDD variants from HBV genotype A, B, C and D, and evaluated the co-variant patterns and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2016
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00439-16